The experiment will be conducted using Canadian white spruce, Picea glauca. On Earth, various portions of a plant can have physically different compositions including different ratios of lignin and cellulose. This will affect the sensitivity of the plants to environmental conditions, to disease and infection and will have an influence on the type of industrial application plants can be used for. It is expected that growth of the trees for 30 days in microgravity will affect their growth rate, composition, tissue organization and gene expression.
Advanced Search Abstract High-throughput and accurate measurements of plant traits facilitate identification of gene function. Along with recent advances in quantitative genomics, there is a growing need for precise quantification of multiple traits in plants.
However, it is difficult continuously to quantify plant adaptive responses to environmental stress responses such as drought because multiple environmental factors are intricately involved in the phenotype.
To solve this problem, we developed an automatic phenotyping system for evaluating the growth responses of individual Arabidopsis plants to a wide range of environmental conditions. RIPPS also records individual rosette size and expansion rate by photographing plants every 2 h. We used RIPPS to establish phenotype evaluation methods for Arabidopsis growth response and water use efficiency under various water conditions, and analyzed the involvement of ABA metabolism in determining water use efficiency.
Plant adaptive responses can vary depending on the developmental stage and target organs Granier and Tardieu To deepen our understanding of the complex mechanisms underlying plant responses to environmental stimuli, it is important to measure plant growth responses quantitatively under different environmental conditions by accurately controlling growth conditions, regulating the timing and strength of the adverse environmental stimuli, and conducting time-course observations of multiple parameters of plant growth.
Quantitative genomics can be facilitated by improving phenotyping accuracy and throughput. RIPPS is an integrated platform that enables us simultaneously to cultivate individual plants in soil pots and automatically to analyze time-dependent changes in plant growth.
Two major benefits are conferred by the automated rotation: RIPPS captures digital images of individual plants in the daytime and during the night-time dark conditions using infrared light-emitting diode LED light peak wavelength nm. Time-dependent changes in plant size and detailed analyses of temporal and spatial effects can be derived and quantified from the time-lapse growth images.
The RIPPS phenotyping platform is operated under precise control of growth parameters including temperature, light, nutrient availability and water conditions.
We used RIPPS to establish quantitative evaluation methods for determining growth responses and water use efficiencies of Arabidopsis plants under different water conditions. These proof-of-concept studies demonstrate that RIPPS accurately facilitates the measurement and evaluation of plant growth responses to precisely controlled hydric environments.
It includes a lighting device with a dimmer control, which work together with the cameras. The system continually rotates up to pots in a stepwise movement, and the pots stopped on the irrigating station are automatically weighed and watered Fig. One step takes approximately 1 min, and one full circuit takes approximately 2 h.
All functions are executed by the operating program on a personal computer Fig. For water deficit experiments, three irrigation mode patterns can be selected to design different experimental conditions, including the timing of water withholding or re-watering, and the soil water content SWC Supplementary Fig.
The amount of water irrigated can be controlled in units of 0. The watering frequency can be freely set by increasing the minimum irrigation amount so as to skip a small amount of watering. Each camera is mounted on a flexible arm of a movable pole on the outer frame of the RIPPS platform, so the camera position is easily adjusted.Soil water content is the primary output of this model.
and is also termed water use. Potential evapotranspiration (Ep) is the water vapor flux under ideal conditions of complete ground cover by plants, uniform plant height and leaf coverage, and an adequate water supply; this is also termed water need.
The risk of chemical leaching. In such plants, a reduction in the rate of growth is apparent in a change in the slope of daily maximum stem-diameter values, which may be due to a lack of water or also to inadequate crop management or changed environmental conditions.
How Does Soil Affect the pH of Water? of tap water. Use pH paper to measure the pH of the tap water, and record that value in your notebook.
Then discard the water (use it to water a plant, etc.). Here is an overview about how to use the RapiTest Digital Soil pH meter in the Science Buddies kit: Press the Power Button to turn the meter.
During the same period of time, the plant requires an amount of water that depends on environmental conditions (light, air humidity, and wind) and on plant traits such as stomatal conductance and leaf area.
Detailed Description of the Experiment. Introduction (written for students) Materials and Methods one might expect plant leaves exposed to drought conditions to have fewer stomata in sunlit environments. stomata density may increase or decrease in response to environmental variation in sunlight and water availability.
Note that since. An Overview of the Experiment, the Water Content of Plants Variation on Environmental Conditions. 2, words. 7 pages. An Overview of the Experiment, Enzymes and the Effect of Concetration. words. 2 pages. An Introduction to the Evolution of Solar .